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mini transblot electrophoretic transfer cell  (Bio-Rad)


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    Structured Review

    Bio-Rad mini transblot electrophoretic transfer cell
    Mini Transblot Electrophoretic Transfer Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+transblot%C2%AE+electrophoretic+transfer+cell+system/Mini-PROTEAN+Tetra+Vertical+Electrophoresis+Cell/pmc12008219-189-22-26
    Average 93 stars, based on 104 article reviews
    mini transblot electrophoretic transfer cell - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Lysis:

    Article Title: Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes.
    Article Snippet: Cell lysis, SDS-PAGE and Western blotting Primary OL or Oli-neu cells were scraped and lysed on ice using modified RIPA buffer (50 mM Tris-HCl pH=7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40 Substitute, Halt® Protease and Phosphatase Inhibitor Cocktails (Thermo Fisher)) and post-nuclear supernatant (PNS) was collected after centrifugation at 1000 x g for 10 min. Proteins were separated by 12% polyacrylamide gels in a Mini PROTEAN 3 system (Bio-Rad) or Novex® NuPAGE® SDS-PAGE 4-12% Bis-Tris gradient gels (Thermo Fisher Scientific) and blotted on ethanol-activated PVDF membranes (Roth) using the Mini TransBlot® Electrophoretic Transfer Cell system (Bio-Rad).

    SDS Page:

    Article Title: Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes.
    Article Snippet: Cell lysis, SDS-PAGE and Western blotting Primary OL or Oli-neu cells were scraped and lysed on ice using modified RIPA buffer (50 mM Tris-HCl pH=7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40 Substitute, Halt® Protease and Phosphatase Inhibitor Cocktails (Thermo Fisher)) and post-nuclear supernatant (PNS) was collected after centrifugation at 1000 x g for 10 min. Proteins were separated by 12% polyacrylamide gels in a Mini PROTEAN 3 system (Bio-Rad) or Novex® NuPAGE® SDS-PAGE 4-12% Bis-Tris gradient gels (Thermo Fisher Scientific) and blotted on ethanol-activated PVDF membranes (Roth) using the Mini TransBlot® Electrophoretic Transfer Cell system (Bio-Rad).

    Western Blot:

    Article Title: Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes.
    Article Snippet: Cell lysis, SDS-PAGE and Western blotting Primary OL or Oli-neu cells were scraped and lysed on ice using modified RIPA buffer (50 mM Tris-HCl pH=7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40 Substitute, Halt® Protease and Phosphatase Inhibitor Cocktails (Thermo Fisher)) and post-nuclear supernatant (PNS) was collected after centrifugation at 1000 x g for 10 min. Proteins were separated by 12% polyacrylamide gels in a Mini PROTEAN 3 system (Bio-Rad) or Novex® NuPAGE® SDS-PAGE 4-12% Bis-Tris gradient gels (Thermo Fisher Scientific) and blotted on ethanol-activated PVDF membranes (Roth) using the Mini TransBlot® Electrophoretic Transfer Cell system (Bio-Rad).

    Modification:

    Article Title: Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes.
    Article Snippet: Cell lysis, SDS-PAGE and Western blotting Primary OL or Oli-neu cells were scraped and lysed on ice using modified RIPA buffer (50 mM Tris-HCl pH=7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40 Substitute, Halt® Protease and Phosphatase Inhibitor Cocktails (Thermo Fisher)) and post-nuclear supernatant (PNS) was collected after centrifugation at 1000 x g for 10 min. Proteins were separated by 12% polyacrylamide gels in a Mini PROTEAN 3 system (Bio-Rad) or Novex® NuPAGE® SDS-PAGE 4-12% Bis-Tris gradient gels (Thermo Fisher Scientific) and blotted on ethanol-activated PVDF membranes (Roth) using the Mini TransBlot® Electrophoretic Transfer Cell system (Bio-Rad).

    Centrifugation:

    Article Title: Dual role of the RNA helicase DDX5 in post-transcriptional regulation of myelin basic protein in oligodendrocytes.
    Article Snippet: Cell lysis, SDS-PAGE and Western blotting Primary OL or Oli-neu cells were scraped and lysed on ice using modified RIPA buffer (50 mM Tris-HCl pH=7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) NP-40 Substitute, Halt® Protease and Phosphatase Inhibitor Cocktails (Thermo Fisher)) and post-nuclear supernatant (PNS) was collected after centrifugation at 1000 x g for 10 min. Proteins were separated by 12% polyacrylamide gels in a Mini PROTEAN 3 system (Bio-Rad) or Novex® NuPAGE® SDS-PAGE 4-12% Bis-Tris gradient gels (Thermo Fisher Scientific) and blotted on ethanol-activated PVDF membranes (Roth) using the Mini TransBlot® Electrophoretic Transfer Cell system (Bio-Rad).



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